Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Pinus thunbergii Parl. Extracts Reduce Acute Inflammation by Targeting Oxidative Stress

doi: 10.1155/2021/7924645

Figure Lengend Snippet: Effect of PTP on oxidative stress in RAW 264.7 macrophages. To measure ROS and SOD concentration, the cells were pretreated with PTP (25, 50, and 100 μ g/mL), DEX (10 μ g/mL), or IBU (10 μ g/mL), then treated with LPS (0.1 μ g/mL). ROS production was detected with a fluorescence microplate reader (Ex. 485 nm; Em. 535 nm) (a), (b). To measure MDA contents, 200 μ L of thiobarbituric acid (TBA) was added to each sample, after which absorbance was measured at 532 nm (c). SOD concentration was measured with an ELISA kit (d). SOD1 and SOD2 production were measured by western blot (e), (f). SOD1 and SOD2 relative density was analyzed using densitometry. All values were reported as the mean ± SD. # indicates a significant difference from the negative control group (# P < 0.05, ## P < 0.01, ### P < 0.001). ∗ indicates a significant difference from the positive control group ( ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001). PTP: Pinus thunbergii Parl.; DEX: dexamethasone; IBU: ibuprofen; CA: caffeic acid.

Article Snippet: ELISA kits for SOD (Cloud‐Clone Corp., Katy, TX, USA) were used according to the manufacturer's protocol.

Techniques: Concentration Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Western Blot, Negative Control, Positive Control

Journal: Addiction & Health

Article Title: Effects of Endurance Exercise Training on Cardiac Dysfunction Induced by Waterpipe Tobacco Smoking

doi: 10.22122/ahj.v11i2.234

Figure Lengend Snippet: The values of blood pressure (BP), levels of plasma cotinine, and levels of malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase, tumor necrosis factor α (TNF-α), and interleukins-1 β (IL-1β), 6, and 10 in the heart in the animal groups

Article Snippet: Moreover, rat superoxide dismutase (SOD) and glutathione peroxidase (GPx) ELISA Kits were purchased from Shanghai Crystal Day Biotech Co. (China).

Techniques: Clinical Proteomics

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Nrf2 Overexpression for the Protective Effect of Skin-Derived Precursors against UV-Induced Damage: Evidence from a Three-Dimensional Skin Model

doi: 10.1155/2019/7021428

Figure Lengend Snippet: The primers of cellular protective genes.

Article Snippet: ELISA assay kits for SOD, CAT, GPX, and GSH were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), while those kits for ROS and MDA were supplied by Nanjing SenBeiJia Bio-Technology Co. Ltd. (Nanjing, China).

Techniques:

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Nrf2 Overexpression for the Protective Effect of Skin-Derived Precursors against UV-Induced Damage: Evidence from a Three-Dimensional Skin Model

doi: 10.1155/2019/7021428

Figure Lengend Snippet: Effect of SKPs on oxidative stress-related biomarkers of 3D-photodamaged skin ( x ¯ ± S ).

Article Snippet: ELISA assay kits for SOD, CAT, GPX, and GSH were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), while those kits for ROS and MDA were supplied by Nanjing SenBeiJia Bio-Technology Co. Ltd. (Nanjing, China).

Techniques: Control, Negative Control, Multiple Displacement Amplification

Journal: International Journal of Molecular Medicine

Article Title: Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway

doi: 10.3892/ijmm.2018.3419

Figure Lengend Snippet: Protein expression of (A) superoxide dismutase (SOD), (B) glutathione (GSH), (C) malondialdehyde (MDA) and (D) 4-hydroxynonenal (4-HNE) in the heart tissue of each group. Data are expressed as mean ± standard deviation (n=3). * P<0.05 or ** P<0.01 vs. control group; # P<0.05 or ## P<0.01 vs. streptozotocin (STZ) group.

Article Snippet: The ELISA kits for SOD, MDA, 4-HNE and GSH were all obtained from Beyotime Institute of Biotechnology.

Techniques: Expressing, Standard Deviation, Control